Growth kinetics of Pseudomonas fluorescens in different media and evaluation of shelf life

Abstract

Biopesticides open up a wide scope for restoration of environment that has been damaged through continuoususe of chemicals. The study focused on assessing the growth kinetics of Pseudomonas fluorescens in various liquid mediaand evaluating their shelf life. Seven media were analysed for their ability to assist the growth and multiplication of thebacterium. Among the various media evaluated, Trypticase Soya broth (TSB) was considered as the effective basal mediumowing to its ability to maintain high population, lesser generation time and lesser cost. Further, it was formulated withvarious amendments like glycerol and PVP (cell protectants), CMC (adjuvant) and Tween 20 (surfactant) and the formulation(TSB+ Glycerol 2%+ PVP 1%+ CMC 0.05%+ Tween-20 0.5%) was found to be promising in sustaining the higherpopulation of Pseudomonas fluorescens up to 240 days of storage.